Background

Obesity is strongly associated with chronic inflammation for which adipose tissue macrophages (ATMs) play a critical role. The objective of this study is to identify monocyte chemoattractant protein-1 (MCP-1) as an important player governing energy balance.

Methods

We evaluated body weight, fat mass, adipocyte size, and energy expenditure as well as core body temperature of MCP-1 knock out mice compared with wild type mice. Adipose tissues were assessed by qPCR, Western blot analysis, and histochemistry.

Results

MCP-1 deficiency augmented energy expenditure by promoting browning in white adipose tissue and brown adipose tissue activity via increasing the expressions of UCP-1, PRDM16, CD137, PGC-1α, NRF1, and tyrosine hydroxylase and mitochondrial DNA copy number.

Conclusions

These results suggested that MCP-1 modulates energy expenditure by impairing browning in adipose tissue.