Because of the obesity acquired during menopause, women are search for tools to lose weight. The weight maintenance through diet is very difficult, when weight recovery occurs and the weight cycling starts. Studies have shown that ovariectomized rats have become hyperphagic and obese. Calorie restriction (CR) brings benefits to the metabolism of the obese through sirtuins. Our aim was to evaluate the effect of the weight cycle on metabolism in an experimental model of menopause.


The approved study (CEA 017/2015) was performed in 24 2-month-old female Wistar rats. The females were submitted to simulated surgery (SHAM) and ovariectomy (OVX). After 2 months, the rats were divided into 3 groups: SHAM, OVX and two cycles of weight (WC). The SHAM group received the commercial feed ad libitum; the cycle was performed with 21 days of chow diet ad libitum and 21 days on 40% CR. The weight was monitored weekly.Following the experimental period, the females were euthanized and the tissues collected for of the protein expression and serum biochemical analyzes.


The OVX females acquired weight associated with changed lipid profile and feature obesity. Biochemical data and body characteristics of WC females offer benefits in metabolism compared to SHAM and OVX groups. Biochemical data and body characteristics of WC females propose advantage in metabolism compared to SHAM and OVX groups. The increase of sirtuins 1 and 4 in the peripheral tissues agree in the increase of the proteins of the electron transport chain, mainly in the adipose tissue in the WC versus OVX animals.


The weight cycle in ovariectomized females favored the obese metabolism suggesting participation of SIRT1 and 4 proteins. The effect of gain and weight loss in the short term suggests that it is more beneficial than being obese.