Background

Afamin is a vitamin-E binding protein that has recently shown to be associated with the genesis and prevalence of metabolic syndrome. Adiponectin and Tumor Necrosis Factor-alpha (TNF-α) are adipokines that have an association with obesity, and metabolic syndrome. This study aims to investigate whether Afamin is correlated with the metabolic syndrome via its association with the adipokines in the obese subjects as they represent insulin resistance (IR) conditions.

Methods

This study is a cross The data and samples of the study were obtained from Qatar Biobank (QBB). The individuals involved in the study were 100 i adults (male and female) with age between 40-60 years old. The data obtained included demographic, anthropometric, clinical and biochemical parameters. All the data were selected randomly based on the inclusion criteria for insulin resistance and MeS. The subjects were grouped into two groups based on the gender and the presence or absence of MeS criteria based on the NECP ATPIII. Group A subjects are having the criteria for metabolic syndrome (MeS). Group B does not have the full criteria of MeS. Serum samples from QBB were used to measure the concentration of afamin, adiponectin, and TNF-α using ELISA assay.

Results

Afamin had no significant correlation with metabolic syndrome criteria components; WC, blood pressure, glucose, HDL-cholesterol level, and TG level. Spearman correlation coefficient demonstrated that afamin neither had a significant correlation with adiponectin nor TNF-α in MeS subjects with p-value=0.215, 0.412, respectively. However, afamin is significantly correlated only with C-peptide, r value=0.370, p= 0.026 among MeS subjects. Increase in C-peptide by 1 ng/ml, increase in afamin by 8.4mg/ml.

Conclusions

There is a significant correlation between C-peptide and afamin in MeS subjects, indicated that afamin is a useful biomarker for insulin resistance. In contrast, afamin has no significant correlation with metabolic syndrome components, adiponectin, and TNF-α.